Composite

Part:BBa_K5223005:Design

Designed by: Chong Yao   Group: iGEM24_SCU-China   (2024-10-01)


Crocin Synthesis System in Nicotiana benthamiana


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1678
    Illegal XhoI site found at 3405
    Illegal XhoI site found at 5753
    Illegal XhoI site found at 7618
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1402
    Illegal BsaI site found at 3129
    Illegal BsaI site found at 5477
    Illegal BsaI site found at 7342
    Illegal BsaI.rc site found at 1681
    Illegal BsaI.rc site found at 3408
    Illegal BsaI.rc site found at 5756
    Illegal BsaI.rc site found at 7621


Design Notes

We plan to achieve heterologous synthesis of crocin through transient transfection, rather than integrating it into the genome, in order to attain higher levels of expression, and we have successfully demonstrated the feasibility of this approach.


Source

Gardenia jasminoides

References